Immobilization of glucose oxidase onto core-shell structure materials Fe3O4NP@SiO2 and testing it’s catalytic activity
Abstract
This work demonstrates herein a method to fabricate magnetic nano enzyme in a form of core-shell structure denoted as Fe3O4NP@SiO2-GOx. The core Fe3O4NP was synthesized from the mixture of Fe2+ and Fe3+ in alkaline medium using co-precipation method, which was coated with SiO2 and then functionalized with (3-aminopropyl) triethoxysilane (APTES). Afterward, Glucose oxidase (GOx) was chemically immobilized onto amine-functionalized Fe3O4NP@SiO2 using glutaraldehyde as a cross-linking agent. Physical characterization by Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD), magnetic saturation, transmission electron microscopy (TEM), and thermal gravimetric analysis (TGA) showed that Fe3O4NP@SiO2-GOx are spherical with diameter in the range of 17 ÷ 28 nm, magnetic saturation value of 34.2 emu/g, catalytic activity of 125 µmol.g-1phút-1 for glucose oxidation, ease of separation from the reaction mixture by applied magnetic field.
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